Although we found that the insulin-induced degradation of IRS-1 [29] and IRS-2 [52] observed in other cell systems does not occur in podocytes, in agreement with previous studies performed in a rat model of streptozotocin-induced diabetes and glomerular endothelial cells [53], we detected a reduction in podocyte IRS-1 content as a consequence of high glucose exposure. Here, IRS2 is linked to diabetes mellitus.