There was no difference in the GPC3 level for the AFP < 20 ng/mL group and the AFP ≥ 20 ng/mL group in 80 HCC patients (6.04 ± 10.68 ng/mL vs. 8.05 ± 11.89 ng/mL, P = 0.47) (Fig. 5B). Here, AFP is linked to hepatocellular carcinoma.