Here we established three cell lines, one epithelial and two mesenchymal, from ascitic fluid of a bladder cancer patient and demonstrated that epithelial cells with surface expression of PD-L1, E-cadherin, CD24, and VEGFR2, transforming phenotype, and E-cadherin-RalBP1 interaction were capable of more rapid tumorigenesis than the mesenchymal cells with constitutively active TGF-β signaling. The gene discussed is CD274; the disease is urinary bladder carcinoma.