Previous research has demonstrated that reduced 2-AG levels are associated with increased risk of outcomes such as post-traumatic stress disorder and major depression,52, 53, 54 while animal studies have demonstrated that increases in 2-AG through pharmacological intervention may reduce anxiety-like behaviours.55, 56 Given the role of DAGLB in the synthesis of 2-AG, it is therefore of great interest that increases in DAGLB expression corresponded with greater reductions in severity, while decreases in DAGLB expression corresponded with lower reductions in severity. Here, DAGLB is linked to major depressive disorder.