Finally, further analysis of the functional profile of Mtb-specific cells revealed that the median fluorescence intensity (MFI) of TNFα in ESAT-6/CFP-10-specific IFNγ+ CD4+ T cells was significantly higher in aTB (regardless of HIV infection), when compared to LTBI (median MFI: 24,920 vs 14,180 in HIV− and 27,720 vs 14,980 in HIV+, respectively) (Figures 4A,B). The gene discussed is CD4; the disease is HIV infectious disease.