The aims of this study were to investigate the expression of total FAK and pFAK-Y397 by immunohistochemistry in pretreatment osteosarcoma tumors of patients with various histologic subtypes, and to investigate correlations of FAK expression with response to chemotherapy, other clinicopathologic characteristics, overall survival (OS), and survival after metastases of all osteosarcoma patients with or without metastasis. This evidence concerns the gene PTK2 and osteosarcoma.