Interestingly, since pancreatic cancer cells including MiaPaCa2 are reported to harbor relatively high basal activity of autophagy, REP1 knockdown in MiaPaCa2 cells revealed a significant reduction in GFP-LC3 puncta formation even in nutrient-complete media, based on quantification results from autophagosome numbers per cell (Figure 3B). This evidence concerns the gene MAP1LC3A and familial pancreatic carcinoma.