To explore the functional effect of REP1 in cancer cells, cells of the pancreatic cancer cell lines MiaPaCa2 and 8988T were transfected with REP1 small interfering RNA to knock down REP1.Compared to treatment with control siRNA, two independent REP1siRNA transfections reduced protein levels of REP1, as determined by immunoblot analysis (Figure 1A). This evidence concerns the gene CHM and familial pancreatic carcinoma.