Although the autophagy markers for autophagosome formation and degradation microtubule-associated proteins 1A/1B light chain 3B (hereafter referred to as LC3) and Sequestosome-1(SQSTM1/p62) were not altered by REP1 knockdown (Figure 3A), when cancer cells stably expressing GFP-LC3 were used for autophagy analysis [20], free GFP levels degraded from GFP-LC3 were enhanced under amino acid-deprivation conditions in Western blotting, and were substantially reduced in REP1-depletionconditions (Figure 3A). This evidence concerns the gene SQSTM1 and cancer.