As a first step we examined glioblastoma cell line GAMG under both cell culture systems for levels of Nanog (green), a marker of pluripotency and found at high levels in the nucleus of GBM cancer stem cells11, and glial fibrillary acidic protein (GFAP) which is an astrocyte marker of differentiation. The gene discussed is GFAP; the disease is glioblastoma.