When the same targets were analyzed in the independent test set of 90 samples (30 LC, 30 HCC, and 30 Recovery), only 2 proteins [fibronectin (FN1) and prothrombin (THRB)] and 5 peptides had an AUROC values  ≥ 0.800 (Supplementary Table S3). This evidence concerns the gene F2 and laryngotracheoesophageal cleft.