Consistently, we conducted luciferase assays in 293 T and THP-1 cells to evaluate the effect of ATG5 rs506027–rs510432 promoter haplotypes on transcription efficiency and confirmed that the rs506027–rs510432 T-G haplotype in ATG5 gene promoter exhibited significantly higher promoter activities compared to the C-A haplotype mutation, suggesting that the sepsis-associated rs506027 and rs510432 polymorphisms played pivotal roles in regulating the gene transcription of ATG5 through the modulation of promoter activity. This evidence concerns the gene ATG5 and Sepsis.