Our data suggested that the protein levels of cleaved caspase-3 and cleaved PARP, and the ratio of Bax/Bcl-2, were significantly increased after bufalin treatment (Fig. 2B,C), indicating that caspase and mitochondrial-mediated apoptotic pathway were involved in bufalin-induced apoptosis of gastric cancer cells. This evidence concerns the gene CASP3 and gastric cancer.