It is important, however, to understand coronary anatomy and the varying degrees of myocardial infarction induced by coronary artery ligation technique in female rodents, as they are often used as a recipient in cell based therapy [18–21]; as real-time polymerase chain reaction for the Y-chromosome-specific sry gene is useful for quantifying the presence and retention of transplanted donor cells, which has been shown to be closely related to therapeutic effects many times, in a model in which the donor (male) cells were transplanted in the damaged female heart. This evidence concerns the gene SRY and myocardial infarction.