In contrast, expression of other Hh target genes, such as Ptc, Collier (Col), and Engrailed (En), did not appear appreciably altered by NS1(Vn) (S1A, S1B, S1E, S1F, S1I and S1J Fig), suggesting that this effect was limited to a specific subset of Hh targets genes (see below, however, regarding the extended effects of the more active NS1 protein from the PR8 strain of influenza). This evidence concerns the gene PTCH1 and influenza.