Considering the CD4 T-cell response, all groups showed a higher frequency of monofunctional T-cells than the polyfunctional, in response to HBHA antigen (LTBI p = 0.002; HIV-LTBI p = 0.002; TB p = 0.024 and HIV-TB p = 0.008 respectively) (Fig 3A). The gene discussed is CD4; the disease is tuberculosis.