Using univariate analysis, CHAC2 expression (P<0.001), the depth of invasion (P<0.001), lymph node metastasis (P=0.003), distant metastasis (P<0.001) and TNM stages (P<0.001) were significantly associated with overall survival rates in gastric cancer patients (Supplementary Table S4). Here, CHAC2 is linked to metastatic malignant neoplasm in the lymph nodes.