To investigate whether 3D growth affects gene transcription, we analyzed the gene expression patterns of BE(2)-C cells grown in 2D, 3D and bioreactor-3D cultures and compared them to tumor tissue samples from three different neuroblastoma patients (German Neuroblastoma Trial cohort30) resembling the characteristics of BE(2)-C cells (stage 4, amplified MYCN, undifferentiated and 1p deletion). Here, MYCN is linked to neuroblastoma.