MAP1LC3B and neoplasm: It has been proposed that tumor cells adopt autophagy as a survival mechanism to cope with metabolic and cytotoxic stress.15, 35, 36 Similarly, spheroid cultures might use this survival strategy, especially in the inner starved zones.33 We examined autophagic flux in our standardized 3D-chip culture using stably transfected BE(2)-C cells expressing the tandem construct mCherry-EGFP-LC3B.