DIO3 and neoplasm: High methylation status of CpG islands in promoters that is common in numerous tumors contributes to the silence of tumor suppressor genes.28 miR-323a-3p is a member of DLK1-DIO3 genomic imprinted miRNA cluster, and increasing evidence reveals that some miRNAs in this cluster are silenced by the upstream IG-DMR and MEG3-DMR.17, 18, 29 Therefore, we performed BSP sequencing, and the results revealed the high methylation level of IG-DMR in BCa cell line (T24, UM-UC3, 5637).