Target genes of predominantly downregulated miRNAs in PMECs at 24 hpc with E. coli were significantly enriched in canonical pathways such as interferon signaling (MX1, ISG15, STAT1, and TAP1); protein ubiquitination (PSMB9, BIRC3, DNAJA1, DNAJC11, PSMD12, TAP1, UBE2R2, USP28, and USP46); glioma invasiveness signaling (DIRAS3, FNBP1, ITGAV, and RHOQ); myc-mediated apoptosis signaling (FAS, IGF1R, SFN, and YWHAG); and Gq signaling (DIRAS3, FNBP1, GNA14, HRH1, NFKBIA, and RHOQ) (Fig. 5b). This evidence concerns the gene ISG15 and central nervous system cancer.