The expression level of TMSB4X in HNSCC was objected to being validated by an RT-PCR and real-time PCR in 35 pairs of the fresh-frozen HNSCC samples (T) and adjacent non-tumor tissues (N), and from 23 NT-paired dataset from the public HNSCC microarray cohort (GSE31056). Here, TMSB4X is linked to head and neck squamous cell carcinoma.