After the comprehensive analysis, we discovered several types of associations (CPPS vs non-CPPS: Osteoc, CEA, C4, C3, TESTO, and CRP; IIIa vs normal: FERR, FSH, and insulin; IIIb vs normal: IgE, ASO, CRP, C3, C4, RF, PSA, and Osteoc; IV vs normal: Osteoc, FSH, and PSA), in which osteocalcin was reproducibly found to be a protective factor in three groups, including CP/CPPS, NIH-IIIb, and NIH-IV prostatitis. The gene discussed is C4A; the disease is prostatitis.