GID8 and colorectal carcinoma: Silencing Twa1 in CRC cells (DLD1 and SW480 cells), which harbor constitutively high levels of nuclear β-catenin due to APC mutations42,43, significantly reduced nuclear β-catenin levels, Wnt reporter activity, and target gene expression (Figure 8A-8C; Supplementary information, Figure S12A-S12C).