To determine the regulation of Twa1 in colorectal carcinogenesis, we first examined several important signaling pathways involved in CRC by dual luciferase reporter assays and observed that Twa1 depletion selectively impaired Wnt signaling in HEK-293 cells (Supplementary information, Figures S2 and S3)23,24,25,26,27,28. The gene discussed is GID8; the disease is colorectal carcinoma.