These findings validated the predicted targets of miR-7641 in different cell lines and supported the possible involvement of miR-7641 in cancers, although the targeting efficiency could vary depending on the cell line; however, several genes, such as RPS16, RNF4, TNFSF10, EMC8, and MSRB3 were downregulated in most of the cell lines and PIGC remained unchanged in all cell lines. This evidence concerns the gene RNF4 and cancer.