During the course of the cell culture process, samples were taken to assess the bioactivity of TRAIL-Trimer using a TRAIL-sensitive human colon cancer derived cell line – COLO205 – by MTT staining (Fig. 2b); as expected, bioactivity increased over time as the production of TRAIL-Trimer continued. Here, TNFSF10 is linked to malignant colon neoplasm.