The results from flow cytometry (i.e, Annexin V-FITC and propidium iodide (PI) staining) determined that ISL at low concentration did not manifest significant effect on apoptosis in breast cancer cells, while the effect of ISL at 25 μM and 50 μM was, at least mostly, through inducing apoptosis (For early apoptosis, P = 0.0038, and P = 0.0031, respectively, and for late apoptosis, P = 0.127, and P < 0.0001, respectively with ANOVA test) (Fig. 1B,C). The gene discussed is ANXA5; the disease is breast cancer.