In Leptospira, it has been proven that the LipL32 stimulated inflammatory responses through TLR2 and the calcium binding cluster (including Asp132, Thr133, Asp164, Asp165, and Tyr178) of LipL32 can regulate the affinity between LipL32 and TLR2 during infection with Leptospira4, 25, 26. This evidence concerns the gene TLR2 and infection.