We measured the expression of the Fasn and the liver‐specific gene targets including Pklr, Pcsk9, and Pnpla3 and found that their expression was significantly (P < 0.05) increased in HCC tumor compared to adjacent noncancerous samples obtained from CB1R+/+ mice (Fig 5D), with much smaller increases noted in corresponding samples CB1R−/− mice. This evidence concerns the gene PNPLA3 and hepatocellular carcinoma.