Genetic or pharmacological loss of GLS1 activity depletes TCA metabolites and slows proliferation of a variety of cancer cell lines in culture (Cheng et al., 2011; Gameiro et al., 2013; Gao et al., 2009; Gross et al., 2014; Le et al., 2012; Seltzer et al., 2010; Son et al., 2013; Timmerman et al., 2013; van den Heuvel et al., 2012; Wang et al., 2010; Yuneva et al., 2012). This evidence concerns the gene GLS and cancer.