After performing specific logistic regression comparing HS to untreated IPF, we found that a model of four Nu.QTM cfnucleosome biomarkers (5mC, HMGB1, H3K27ac, and H3K9ac), reached an AUC of 0.93 in a ROC curve analysis (80% specificity and 91% sensitivity), which holds promise for use in clinical practice. Here, HMGB1 is linked to idiopathic pulmonary fibrosis.