The TNF and CCL2 levels at 16 h of simulation were similar to those observed in the cultures of monocytes isolated from patients with sepsis and challenged with 5 ng/ml LPSs for 16 h (Figures 6D,E) reinforcing the idea that this model is able to reproduce the ET exhibited by cultured monocytes from patients with sepsis when they are challenged with LPSs, and that it could be used to determine the tolerance status of the monocytes of these patients. The gene discussed is CCL2; the disease is Sepsis.