Herein, we set out to further characterize in vitro and in vivo the role of type I IFN for murine hepatic iNOS regulation by using the cellular model of IFNβ-stimulated hepatoma cells (Hepa1-6, Hepa56.1D) and hepatocytes and by investigating murine acetaminophen (paracetamol, APAP)-induced sterile liver inflammation in the context type I IFN receptor-deficient mice. The gene discussed is NOS2; the disease is inflammation.