To evaluate whether S. Typhimurium infection induces the differentiation of Treg cells in these tissues, C57BL6 mice we orally infected with 1 × 105 CFUs and 96 h post-infection were measured the amount of CD4+CD25+FoxP3+ and CD4+CD25+FoxP3− T cells in situ (as described in Figure S3 in Supplementary Material). This evidence concerns the gene FOXP3 and infection.