To further confirm the effect of TMZ on GADD45A expression in GBM cells, we treated four different types of GBM cell lines, including two TMZ-sensitive cell lines (U87 and U373), one natural TMZ-resistant cell line (T98) and one adapted TMZ-resistant cell line (TR-U373), with 25 μg/ml TMZ for 96 hours and analyzed GADD45A expression. The gene discussed is GADD45A; the disease is glioblastoma.