As with the microarray profiling data, HEPH was noticeably up-regulated in G9a-knockdown breast cancer cells (MCF-7, MDA-MB-231, ZR-75-30, S1, SK-BR-3 and MDA-MB-435) compared with the control (Fig. 2b and Supplementary Fig. 1a, 6a, 9). This evidence concerns the gene HEPH and breast cancer.