Comparison of the gene expression profile using Affymetrix genechip human genome U133 plus 2.0 and artificial neural network (ANN) analysis of the patient’s sample with 36 tumor samples (12 rhabdomyosarcoma/RMS, 6 Ewing’s sarcoma/EWS, 14 neuroblastoma/NB and 4 Wilms’-Tumor/WT specimen) revealed that the patient sample (test as grey) was closely clustering with neuroblastoma (Fig. 3a; Additional file 1: Table S1). The gene discussed is EWSR1; the disease is neuroblastoma.