For example, only one of the replicates supported the presence of the known fusion genes KCTD5-AC141586.5 (10 spanning reads) and OAZ1-KLF2 (4 spanning reads) in ALL 668, while the TPM4 − KLF2 (12 spanning reads) was identified only in one AML 1867-RD library (Additional file 3: Figure S8), indicating that low coverage may indeed impair fusion gene detection. Here, TPM4 is linked to acute lymphoblastic leukemia.