Finally, we illustrate the method on a microRNA gene expression study in neuroblastoma to find differentially expressed genes between MYCN amplified and MYCN single copy tumor samples [4], and on a mRNA gene expression study in neuroblastoma to detect differentially expressed prognostic genes between patients with higher risk of death from disease or higher risk of relapse or progression and patients with low risk [5]. The gene discussed is MYCN; the disease is neuroblastoma.