The best CpGs identified by the ROC analysis were used to create an algorithm of choice to correctly discriminate OSCC and HGSIL, with the exception of MIR137HG, which was previously shown to be aberrantly methylated also in oral lichen planus (OLP) [15], and PAX1 which was used as an informative marker for a patent related to prognosis prediction in Head and Neck Squamous Cell Carcinoma (HNSCC) [20]. Here, PAX1 is linked to head and neck squamous cell carcinoma.