Further, mitochondrial membrane potential loss, elevation of cytosolic cytochrome c and Bax levels, decrease in Bcl-2 levels, enhanced caspase-9 and -3 activities confirm the apoptosis-inducing potential of compound 9f in MCF-7 breast cancer and HL-60 leukemia cell line via mitochondria-mediated intrinsic pathway. Here, CYCS is linked to leukemia.