These transcriptional measurements were complemented by biochemical quantification of collagen, which is widely accepted as the gold-standard endpoint for studies of lung fibrosis (30) (here measured by the Sircol assay), where whole lung samples from VEGF-Tg mice exhibited a 40.1% reduction in the degree of collagen accumulation (P = 0.0073, Figure 4B). Here, VEGFA is linked to pulmonary fibrosis.