To rule out that degradation of the total RNA isolated from the archival samples impairs accurate quantitation we also determined BRD4 mRNA levels in a set of fresh frozen plexiform neurofibromas (n = 7), atypical neurofibromas (n = 4) and MPNST (n = 11) (Fig 1B). This evidence concerns the gene BRD4 and plexiform neurofibroma.