Since an increase in antigen-experienced memory CD4+ T cells was observed in our CTS patients and since increased T cell reactivity to peripheral myelin proteins has been reported in patients with immune-mediated neuropathies31, 32, we next assessed the frequency of antigen specific cytokine secreting PBMCs using an enzyme-linked immunospot (ELISPOT) assay. This evidence concerns the gene CD4 and carpal tunnel syndrome.