Because cerebral ischemia is one of the most frequent causes of brain injury [24], then to study the role of uPA and uPAR in the injured brain we used an in vitro model of hypoxia in which neurons are exposed to oxygen and glucose deprivation (OGD) conditions, and an in vivo model of cerebral ischemia in which the middle cerebral artery is occluded (MCAO) with a suture during different periods of time. Here, PLAU is linked to brain ischemia.