To our knowledge, this is the first paper revealing that GTE alone can induce the biomarkers of beige adipose tissue and the multiple pathways of browning that involve either PPAR-γ, PRDM-16, FGF-21, BMP-7, or TLE-2 associated with a prevention of HED-induced obesity in SD rats. Here, FGF21 is linked to obesity due to melanocortin 4 receptor deficiency.