Because of the query of CD133 as a GSC marker2, 10, 35, we combined CD133 with CD1536 and NESTIN2, 37, 38 as GSC markers to improve the validity of our study, and GFAP7, 11, 27 was used as the differentiated marker of glioma cells according to traditional studies. The gene discussed is PROM1; the disease is central nervous system cancer.