In another study, activation of the S6K1 pathway by nutrients or by prolonged insulin treatment led to insulin resistance due to increased IRS-1 serine phosphorylation and led to a reduction in IRS-1 function, as well as impaired activation of the PI3K/Akt pathway, thereby creating a negative feedback loop on insulin action in adipose tissue43. The gene discussed is IRS1; the disease is Insulin resistance.