RA treatment led to a prominent decline in Calr as well as p53 expression, indicating that reduced p53 was directly associated with the expression of Calr. To support this, cell infection was performed to specifically upregulate Calr, leading to the increased expression of p53. Importantly, Nkx2.5 was not affected by Calr or p53 expression. The gene discussed is CALR; the disease is infection.