From the above, the present study revealed that ARS could significantly increase the CRF, ACTH and CORT levels, and this effect could be retrained by DZP and SAG (3.6 mg/kg).Furthermore, SAG at the dose of 1.8 mg/kg could also reverse the increase of CRF and CORT caused by ARS. This evidence concerns the gene POMC and Axenfeld-Rieger syndrome.