After 2 weeks of TAC, a remarkable reduction of pAKT S473 was found in the LV tissues of WT TAC mice compared to WT sham (Fig. 4B–D), which is consistent with previous studies (Kemi et al., 2008; Borghi & Tartagni, 2012); however, this TAC‐induced reduction in pAKT S473 was prevented in VCP TG mice (Fig. 4B–D). The gene discussed is VCP; the disease is persistent truncus arteriosus.