The expression of specific genes (Robo1, Ntn1, Nrp1, Ntng1, Gsk3b, Rac1, Rock2, Clf2, Efnb3) from this pathway were quantitated by qPCR, and five genes (Robo1, Ntn1, Nrp1, Ntng1, Efnb3) were found to be down regulated in NSCs from diabetic pregnancy when compared to the control (Figure 4), suggesting that hyperglycemia deregulates axonal guidance signaling. The gene discussed is ROCK2; the disease is Hyperglycemia.