The Bond-III platform required high levels of 22C3 antibody concentrate (2 incubations of 60 minutes at 1:10 dilution) to reach the gold standard of PD-L1 IHC 22C3 pharmDx on the Dako ASL48 platform on tonsil and the three NSCLC samples (see S5 Fig). This evidence concerns the gene CD274 and non-small cell lung carcinoma.